gENE oNTOLOGY
Gene ontology is broken down into 3 groups that describe the categories that define a gene and what is does in the cell. These terms are biological processes, cellular components and molecular function. It is comprised of numerous databases and provides insight into what role the gene or protein has within the cell.
This Ontology was done using GO= Gene Ontology Consortium [1].
This Ontology was done using GO= Gene Ontology Consortium [1].
Discussion and Results
For human Gene Ontology, there is a large amount of biological processes related to keratinocytes and cell division.
Biological Processes
regulation of cyclin-dependent protein serine/threonine kinase activity (GO:0000079) release of cytochrome c from mitochondria (GO:0001836) keratinocyte development (GO:0003334) negative regulation of protein kinase activity (GO:0006469) apoptotic process (GO:0006915) signal transduction (GO:0007165) intrinsic apoptotic signaling pathway in response to DNA damage (GO:0008630) regulation of epidermal cell division (GO:0010482) regulation of epidermal cell division (GO:0010482) negative regulation of keratinocyte proliferation (GO:0010839) keratinocyte differentiation (GO:0030216) positive regulation of cell growth (GO:0030307) keratinization (GO:0031424) negative regulation of cysteine-type endopeptidase activity involved in apoptotic process (GO:0043154) skin development (GO:0043588) positive regulation of epidermal cell differentiation (GO:0045606) positive regulation of epidermal cell differentiation (GO:0045606) positive regulation of protein export from nucleus (GO:0046827) regulation of cell cycle (GO:0051726) membrane organization (GO:0061024) establishment of skin barrier (GO:0061436) negative regulation of protein serine/threonine kinase activity (GO:0071901) intrinsic apoptotic signaling pathway (GO:0097193) positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway (GO:1900740) positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway (GO:1900740) |
Cellular Components
extracellular region (GO:0005576) extracellular space (GO:0005615) nucleus (O:0005634) cytoplasm (GO:0005737) cytosol (GO:0005829) cytoplasmic vesicle membrane (GO:0030659) extracellular exosome (GO:0070062) |
Molecular Function
protein binding (GO:0005515) protein kinase C inhibitor activity (GO:0008426) protein kinase binding (GO:0019901) protein domain specific binding (GO:0019904) phosphoprotein binding (GO:0051219) |
Since I will be using C.elegans as my model organism, I followed the ontology for C.elegans. As I have shown in 14-3-3sigma, the GO Terms are very similar to what 14-3-3 sigma does within the cell.
Biological Process
zygote asymmetric cell division (GO:0010070) first cell cycle pseudocleavage (GO:0030590) pseudocleavage (GO:0030588) polarity specification of anterior/posterior axis (GO:0009949) specification of axis polarity (GO:0065001) cytoplasmic translational initiation (GO:0002183) cell junction maintenance (GO:0034331) positive regulation of histone methylation (GO:0031062) |
Cellular Components
phosphoribosylaminoimidazolesuccinocarboxamide synthase activity (GO:0004639) phosphoribosylaminoimidazole carboxylase activity (GO:0004638) phosphoribosylamine-glycine ligase activity (GO:0004637) coenzyme transporter activity (GO:0051185) RNA polymerase I regulatory region DNA binding (GO:0001013) phospholipase D activity (GO:0004630) |
Molecular Function
cortical endoplasmic reticulum (GO:0032541) stress fiber (GO:0001725) methylosome (GO:0034709) transcription factor TFIIF complex (GO:0005674) DNA replication factor C complex (GO:0005663) |
References:
[1] Term Enrichment Service. (n.d.). Retrieved March 28, 2015, from http://amigo.geneontology.org/rte